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Mar 23, 2018 Western michigan university athletic budget, write my research paper -

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Amylase vs Starch vs Temp vs Buffers Lab Report. “Effects of Amylase reaction time when breaking down starch. ” Experiment Goal: The goal of our experiment was to understand the similarities in digestion by finding out how long it takes for the amylase enzyme, found in saliva, to break down our substrate, starch. Hypothesis: While understanding that starch is broken down by our saliva (amylase enzyme) we predict that the higher the michigan athletic budget, concentration of the enzyme the faster the breakdown of the starch. Materials: Brightly colored test tube rack, kim wipes, beaker for trash 1. 5 ml microcentrifuge tubes. Test plate Micropipetters and tips DI water Buffer solution … 4. 5 to 8. 8 I2Kl (grams iodine) Starch solution Enzyme (amylase) 80 degree Celsius water (HOT) Floating test rack Procedure: While controlling the amount of starch and county freshers 2013, the amount of buffer we use with a pH of 5. Michigan Athletic! 8, we want to investigate how changes in enzyme concentration affect reaction rates. First we put 500 ml of amylase from 0. 2% solution (provided by Michael Bunch) into an experimental tube.

We then put 3 drops of Iodine into florida campus a test plate (the color is brown). Need essay sample on Amylase vs Starch vs Temp vs Buffers Lab Report ? We will write a custom essay sample specifically for you for michigan athletic only $12.90/page. While ready to time our reaction, we added 250 ml of starch solution into the mixture of buffer and amylase. The reaction had started instantly. We first timed the reaction at 20 seconds. At 20 seconds, we removed a 25 ml sample of the mixture and dropped it into one of the test sights of campus, iodine. The color of the brown iodine turned immediately purple. Traces of purple in the iodine and amylase mixture indicate that the amylase did not have enough time to break down the starch completely. We tried this pattern of every 20 seconds for michigan up to three minutes. The amylase was not breaking down the starch. Because no reaction after too much time, we increased the amylase solution to 0. 5% and started our experiment over.

After resetting our experiment back up we then made our solution of amylase and starch and started our timing intervals at 10 seconds. Of Saskatchewan Meaning! The color of the western athletic budget, iodine was brown after only 10 seconds. This indicated that upping the amylase concentration to 0. Jay Lokken Of Wisconsin Stout! 5% made the reaction faster, but not too fast. Since the michigan athletic budget, reaction was too fast for lancaster university county college 2013 complete observation we diluted the 0. 5% solution so that it could slow down the reaction time. Western Michigan Athletic Budget! We tried a 200/800 enzyme to buffer or 0. 1% of the 0. 5% solution.

After mixing the of wisconsin, solution and timing again we discovered that 10 seconds was still too fast and that the iodine was again brown. With another diluting solution in mind we diluted the 0. 5% dilution to a 100/900 ml solution and again after 10 seconds STILL TOO FAST. Another appropriation was made with a 60/940 ml solution, and to no surprise the reaction was still too fast. While running out of michigan university athletic budget, options we finally decided to try one last solution of union ky ulearn, a 20/980 ml solution and found that after 10 seconds the iodine was finally purple. We then kept timing with a 10 second interval, and at 20 seconds took another 25 ml sample and the starch was resolved and the iodine was then brown. While finding a way to slow down the reaction 10 seconds we found that a 20/980 or 1/50 enzyme to buffer it took 20 seconds for university budget the amylase to break down the motto meaning, starch. Now with one more curiosity we tried a 10/490 solution and found that 10 seconds was purple, 20 seconds was purple, and that the starch was resolved at 30 seconds in brown iodine. Conclusion: In this part of the experiment we learned that our hypothesis was correct and that the concentration was extremely important and needed to be changed drastically with dilutions to be able to see the effect in slow motion. PART II of enzyme vs starch experiment: After becoming quite good at being able to test enzyme to starch solutions we were to find out whether the pH of the buffer had an effect to the reaction time.

Hypothesis: With learning that the amylase concentration is western athletic, a big factor in the experiment we predict that the ohio southern, pH will have the same effect and that the lower the pH the michigan university athletic budget, faster the reaction time. Procedure: With the same experiment set up and mein essay, design we controlled the solution with what we left off and had a 10/990 enzyme to buffer solution, but changed the pH of the buffer. We then started out with a 4. 5 pH level and started mixing and timing. We timed in western michigan athletic, 5 second intervals and lancaster, found that the starch lasted for 20 seconds. After learning that pH had a fast reaction time with an acidic pH level we decided to try a base pH level buffer of 8. 8. We then set up our products the same as the others with a test tube with 490 ml of buffer, then adding 10 ml of amylase solution, then finally adding 250 ml of starch.

We then started timing in western athletic budget, 10 second interval. Of Central Photos! The reaction time of the starch breakdown was well past 4 minutes, so we decided to end this round of tests. With one more pH level to test, our hypothesis looked correct. Western Michigan Athletic Budget! With a 7. 4 pH level we used 20 second intervals with the exact same solution and university motto meaning, found that it took starch a total of 4 minutes to michigan university, totally break down. Conclusion: In conclusion to part II we discovered that our hypothesis was correct and that the county 2013, more acidic the pH the faster amylase is able to break down starch. Part III of Amylase vs Starch experiment: With the western michigan athletic, new understanding of amylase, starch, and pH level reactions the county ford, last variable we wanted to test was the effect temperature had on the reaction time. Hypothesis: Our hypothesis was that the hotter the water the faster the reaction time of starch breakdown. Procedure: Starting with 990 ml of 7. Western University Athletic Budget! 4 pH buffer, 10 ml of enzyme, and 250 ml of starch floating in a floating test rack in a water bath of lancaster university college freshers 2013 ford, 80 degree Celsius heater water we let the experiment elements heat up for two minutes before we started mixing and western athletic budget, timing our solutions. After letting the elements heat up we mixed our solution and started timing with 10 second intervals and found that once the starch was added to ohio, iodine the reaction was profusely purple.

We timed until 4 minutes and discovered that the starch never broke down. Conclusion: After running part III of this experiment we learned that our hypothesis was wrong, and actually acted the exact opposite. Heat slows down the western, enzymes ability breakdown of union college ky, starch immensely. We were unable to get the michigan, starch to break down with so little time. Stout! The cooler the environment the faster the amylase enzyme is able to break down starch.

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Biology/The Human Heart term paper 895. Click Here For Research Papers Online! The Human Heart. Dorland's Illustrated Medical Dictionary defines the heart. as the viscus of western michigan university budget, cardiac muscle that maintains the. circulation of the blood. It is college ky ulearn ky, divided into four. cavities; two atria and two ventricles. The left atrium. receives oxygenated blood from the lungs.

From there the. blood passes to the left ventricle, which forces it via the. aorta, through the western university athletic arteries to supply the tissues of the. body. The right atrium receives the blood after it has. passed through the tissues and has given up much of its. oxygen.

The blood then passes through the right ventricle. into the lungs where it gets oxygenated. There are four. major valves in the heart; the left atrioventricular valve. (also known as the lancaster university county college freshers ford mitral or bicuspid valve), the right. atrioventricular valve (tricuspid), aortic valve, and the. pulmonary valve.

The heart tissue itself is nourished by. the blood in the coronary arteries.2. Position of the Heart Within the Body: The heart is placed obliquely in the chest. The two atria. are directed upwards and backwards to athletic budget, the right and are at. the level of the photos fifth through the eight dorsal vertebrae. The apex of the heart points downwards and forwards to the. left and corresponds to the interspace between the fifth and.

sixth ribs, two inches below the left nipple. Its atrial. border corresponds to a line drawn across the sternum on a. level with the upper border of the third costal cartilage. Its lower border (apex) corresponds to a line drawn across. the lower end of the same bone, near the xiphoid process. Its upper surface is rounded and western michigan university athletic budget convex, directed upwards. and forwards, and jay lokken university formed mainly by the right ventricle and. part of the left ventricle. The posterior surface of the. heart is flattened and rests upon michigan athletic budget, the diaphragm muscle.

Of. its two borders, the right is the longest and thinnest, the. left is shorter but thicker and round. In an adult, the heart measures about five inches in. length, three and a half inches in the broadest part of its. transverse diameter, and two and a half inches in its.

antero-posterior. The average weight in the male varies. from ten to twelve ounces. In the female, the average. weight is eight to ten ounces. The heart will continue to. grow in size up to an advanced period of life.

This growth. is more obvious in men than in women.3. Circulation of Blood in an Adult: The heart is subdivided by a longitudinal muscular septum. into two lateral halves which are named right and left. according to their position. A transverse muscle divides. each half into two cavities. The upper cavity on each side.

is called the atria/auricle, and the lower side is called. the ventricle. The right atrium and ventricle form the. venous side of the heart. Dark venous blood is pumped into. the right atrium from the entire body by the superior (SVC) and inferior vena cava (SVC), and the coronary sinus. From. the right atrium, the blood passes into the right ventricle. and from the right ventricle, through the pulmonary artery. into the lungs.3 Once the blood becomes.

oxygenated/arterialized by its passage through the union college ky lungs, it. is returned to the left side of the heart by the pulmonary. veins which open into the left atrium. From the left. atrium, the blood passes into the left ventricle where it is. distributed by the aorta and its subdivisions through the. Morphology of Each Heart Chamber: The right atrium is a little longer than the western michigan university left. Its. walls are also somewhat thinner than the left.

The right. atrium is capable of containing about two ounces of fluid. It consists of two parts, a principle cavity/sinus, and an. appendix auriculae. The sinus is a large. quadrilateral-shaped cavity located between the IVC and of saskatchewan the. SVC. Western University! Its walls are extremely thin and university campus scholarships are connected on the. lower surface with the right ventricle and internally with.

the left atrium. Western Michigan University Budget! The rest of the right atrium is free and. unattached. The appendix auricle is a small conical. muscular pouch. It projects from the freshers sinus forwards and to. the left side, where it overlaps the root of the pulmonary. There are four main openings into the right atrium; the. SVC, IVC, coronary sinus, and the atriculo-ventricular.

opening. The larger IVC returns blood from the lower half. of the university athletic body and opens into the lowest part of the right. atrium, near the ohio university southern campus septum. Athletic! The smaller SVC returns blood from. the upper half of the body and opens into the upper and.

front part of the right atrium. The coronary sinus opens. into the right atrium between the IVC and. auriculo-ventricular opening. It returns blood from the. cardiac muscle of the heart and scholarships is protected by a. semicircular fold of the lining membrane of the atrium, called the coronary valve. The auriculo-ventricular opening. is the large oval aperture of communication between the.

right atrium and ventricle. There are two main valves. located within the western michigan university athletic budget right atrium; the county freshers Eustachian valve and. the coronary valve.3 The Eustachian valve is located. between the western university athletic budget anterior margin of the IVC and the. auricule-ventricular orifice.

It is semilunar in form. The. coronary valve is a semicircular fold of the lining membrane. of the right atrium, protecting the orifice of the union college ky ulearn coronary. The right ventricle is triangular-shaped and extends from. the right atrium to near the apex. Its anterior surface is.

rounded and convex and forms the larger part of the front of. the heart. Its posterior surface is flattened, rests on the. diaphragm muscle, and forms only a small part of this. surface. University Athletic Budget! Its inner wall is formed by the partition between. the two ventricles, the university stout septum, and bulges into the cavity.

of the right ventricle. Superiorly, the ventricle forms a. conical structure called the infundibulum from which the. pulmonary artery arises. Michigan University! The walls of the right ventricle. are thinner than those of the left ventricle. The thickest. part of the wall is at the base and it gradually becomes. thinner towards the apex.

The cavity can contain up to two. ounces of fluid. There are two openings in university of wisconsin stout, the right ventricle; the. auriculo-ventricular opening and the opening of michigan university budget, the. pulmonary artery. The auriculo-ventricular opening is the. large oval opening between the right atrium and the right. ventricle. The opening is university meaning, about an inch in diameter. It is.

surrounded by a fibrous ring, covered by the lining membrane. of the heart (endocardium), and is larger than the opening. between the left atrium and the left ventricle. It is. protected by the tricuspid valve. The opening of the.

pulmonary artery is round and is situated at the top of the. conus arteriosus, close to western michigan university athletic, the septum. It is on the left. side and is in front of the auriculo-ventricular opening. It is protected by the semilunar valves.3. There are two main valves associated with the college right. ventricle; the tricuspid valve and the semilunar valves. The tricuspid valve consists of three segments of budget, a. triangular shape, formed by the lining membrane of the heart.

(endocardium). They are strengthened by a layer of fibrous. tissue and muscular fibers.1 These segments are connected. by their bases to the auriculo-ventricular orifice, and by. their sides with one another, so as to form a continuous. membrane which is attached around the margin of the. auriculo-ventricular opening. Their free margin and. ventricular surfaces are attached to many delicate tendinous.

cords called chordae tendinae. The central part of each. valve segment is thick and strong while the lateral margins. are thin and indented. The chordae tendinae are connected. with the adjacent margins of the main segment of the valves. The semilunar valves guard the opening of the pulmonary. artery. They consist of three semicircular folds formed by. the endothelial lining of the heart and are strengthened by.

fibrous tissue. They are attached by their convex margins. to the wall of the artery at its junction with the. ventricle. The straight borders of the valve are unattached. and are directed upwards in the course of the vessel, against the motto sides of which they are pressed during the. passage of blood along its canal. The free margin of each. valve is somewhat thicker than the rest of the valve and is.

strengthened by a bundle of tendinous fibers. University Athletic Budget! During the. passage of blood along the pulmonary artery, these valves. are pressed against the sides of its cylinder. During. ventricular diastole (rest), when the current of blood along. the pulmonary artery is checked and partly thrown back by. its elastic walls, these valves become immediately expanded. and close the entrance of the tube. 3. The left atrium is of central photos, smaller but thicker than the right.

atrium. Michigan University Athletic! It consists of two parts; a principle cavity/sinus. and an appendix auriculae. The sinus is cuboidal in form. and is covered in the front by the pulmonary artery and the. aorta. Internally, it is separated from the right atrium by.

the septum auricularum. Behind the sinus on motto, each side, it. receives the pulmonary veins. The appendix auriculae in the. left atrium is narrower and more curved than the same. structure in the right atrium. Its margins are more deeply.

indented, presenting a kind of foliated appearance. Its. direction is forwards towards the right side, overlapping. the root of the western michigan university athletic budget pulmonary artery. There are two main openings in the left atrium; the. openings of the four pulmonary veins and the.

atrial-ventricular opening. Two of the campus photos four pulmonary veins. open into western university athletic budget, the right side of the atrium and two open into the. left side. The two veins on the left exit into the atrium.

through a common opening. None of the pulmonary veins have. valves. The atrial-ventricular opening is the large oval. opening of university stout, blood flow between the atrium and the ventricle. It is smaller than the same opening between the western right atrium. The left ventricle is longer and more conical shaped than. the right ventricle. It forms a small part of the left side. of the anterior surface of the heart and a large portion of.

the posterior surface. It also forms the apex of the heart. because it extends beyond the mein essay help right ventricle. Its walls. are nearly twice as thick as those of the right ventricle. They are thickest in the broadest part of the ventricle, becoming gradually thinner towards the university athletic base and also towards. the apex, which is the thinnest part of the left ventricle. There are two main openings in the left ventricle; the. atrial-ventricular opening and the aortic opening.

The. atrial-ventricular opening is located behind and to the left. side of the aortic opening. The opening is a little smaller. than the same opening between the right atrium and. ventricle. Its position corresponds to the center of lancaster, the.

sternum. It is surrounded by a dense fibrous ring and is. covered by the lining membrane of the heart and is protected. by the mitral valve. The circular aortic opening is located. in front of and to the right side of the atrial-ventricular. opening from which it is separated by western michigan university one of the segments of.

the mitral valve. The opening is protected by the semilunar. There are two valves located within the left ventricle; the. mitral valve and the semilunar valve. The mitral valve is. attached to the circumference of the atrial-ventricular. opening in the same way that the tricuspid valve is attached. on the opposite side of the heart. Jay Lokken Of Wisconsin Stout! The valve contains a few.

muscular fibers, is western university, strengthened by fibrous tissue, and is. formed by the lining of the heart (endocardium). It is. larger, thicker, and ohio southern campus stronger than the tricuspid, and. consists of two segments of university budget, unequal size.

The mitral valves. are connected to many chordae tendonae. Lieblingsbuch Help! Their attachment is. the same as on the right side except they are thicker, stronger, and western michigan athletic budget less numerous. The semilunar valves surround. the aortic opening. They are similar in structure and mode. of attachment to those of the pulmonary artery. However, they are larger, thicker, and stronger than those of the.

right side. Between each valve and the cylinder of the. aorta is a deep depression called the sinuses of university stout, Valsalva. The depressions are larger than those at the root of the. Figure 1: a. Cross sectional view of the heart. Western Michigan Budget! b. Top.

view of the heart showing the four valves. Histology of the Layers of the Heart: The heart and its vessels are surrounded by a conical. membranous sac called the pericardium. The pericardial sac. is composed of two layers; the parietal pericardium and the.

visceral pericardium with the space in-between the university motto two being. called the pericardial cavity. The parietal pericardium is. composed primarily of compact fibrocollagenous tissue along. with elastic tissue. It is a fibrous membrane of loose. irregular connective tissue that is lined internally by a. mesothelium which is essentially simple squamous epithelium. The visceral pericardium forms the internal lining of the.

pericardium and reflects over the outer surface of the. heart. This reflection forms the outer layer of the. epicardium. The visceral pericardium is also composed of. compact fibrocollagenous tissue with elastic tissue but, is. smooth mesothelium. The pericardial cavity is located. between the parietal and visceral pericardium and contains. small amounts of western athletic, serous fluid.

The heart tissue itself can be subdivided into three. layers; (from the outside in) epicardium, myocardium, and. endocardium. The epicardium is the outermost layer of the. heart and consists of campus scholarships, a loose connective tissue of. fibroblasts, collagen fibers, and adipose tissue. It. contains a stroma which houses coronary arteries and veins.

that are surrounded by a layer of fat. These coronary. branches penetrate the myocardium. The myocardium contains the main muscle mass of the heart. and is composed primarily of striated muscle cells.

Each of. the cardiac muscle cells contain one central elongated. nucleus with some central euchromatin and some peripheral. heterochromatin. University Athletic Budget! The two atria have a very thin myocardial.

layer which increases greatly in ohio university campus, thickness as you go from. the atria to the right ventricle and into the left. ventricle. The outer surface of the myocardium, next to the. epicardium, is university budget, not composed of smooth muscle but is very. smooth in texture. The inner surface of the university county freshers ford myocardium is. rough and western michigan university is raised into trabeculations. The ventricular. papillary muscles, which are for lieblingsbuch essay the attachment of the.

chordae tendinae, are extensions of the myocardium even. though they are covered by endocardium. The outer layer of. the myocardium is superficial bulbospiral and swirls around. the ventricle in a clockwise fashion. The middle layer is. circular muscles that are the ventricular constrictors.

The. inner layer, which is deep bulbospiral, swirls around the. ventricle in western michigan university, a counterclockwise fashion. The layer underneath the university of saskatchewan myocardium is known as the. enodcardium. It contains a continuous smooth endothelial.

layer that covers all the inner surfaces of the heart, including the valves. Western University Athletic Budget! The outer layer of the endocardium, underneath the myocardium, is irregularly arranged. collagenous fibers that may contain Purkinje fibers/cells. The inner part of the endocardium contains more regularly. arranged collagen and university motto elastic fibers than the outer layer. Some myofibroblasts are present in the endocardium which is. thicker in the atria than in the ventricles. There is a. subendothelial component of the endocardium underneath the.

endothelium. The component contains fibroblasts, scattered. smooth muscle cells, elastic fibers, collagen fibers, and an. amorphous ground substance that contains glycoproteins and. The valves of the heart are attached to the cardiac. skeleton and consist of chondroid (a material resembling. cartilage). The base of each valve is supported by western michigan a. fibrocollagenous ring.

Each valve also has a dense. fibrocollagenous central plate that is covered by simple. squamous epithelium. Jay Lokken University! Chordae tendonae connect with the. valves at the edge of each cusp as well as underneath each. cusp at one end and they attach to papillary muscles in the. ventricles at the other end. Western Michigan Budget! Endocardial endothelium.

completely covers the ohio university southern campus scholarships papillary muscles, valves, and the. chordae tendonae. The junctions between the cusps of each. valve are known as commissures. The conducting system of the heart consists of michigan athletic budget, four main. components; the sinuatrial node (SA), the of central photos atrioventricular.

node (AV), the bundle of his, and the Purkinje fibers/cells. All the parts of this conducting system are composed of. modified cardiac muscle cells. The SA node is western budget, located in. the right atrium, at the point where the superior vena cava. enters. Jay Lokken University Of Wisconsin! The small muscle fibers of the SA node contain a. central nodal artery and desmosomes.

The muscle fibers do. not contain intercalated discs. The AV node is located in. the medial wall, in front of the opening of the coronary. sinus and above the michigan university athletic budget tricuspid ring. Its small muscle fibers. are more regularly arranged than those of the SA node. The. AV node contains a rich nerve and blood supply.

The bundle. of his has a right (single bundle) and a left (branched. bundle) bundle branch located underneath the endocardium. It is histologically similar to the other components of ky, the. conducting system. Michigan University Budget! The Purkinje fibers/cells can be found. in clusters of about six cells which are located under the. endocardium in the ventricles. The cytoplasm of Purkinje. fibers appears pale under the microscope and jay lokken university contains many.

Physiology of the Heart: The principle function of the heart and circulatory system. is to provide oxygen and western michigan nutrients and to remove metabolic. waste products from tissues and of saskatchewan meaning organs of the body. The. heart is the pump that provides the energy necessary for. transporting the budget blood through the circulatory system in. order to facilitate the exchange of oxygen, carbon dioxide, and other metabolites through the thin-walled capillaries. The contraction of the heart produces changes in pressures. and flows in the heart chambers and blood vessels. The.

mechanical events of the cardiac cycle can be divided into. four periods; late diastole, atrial systole, ventricular. systole, and early diastole.6. In late diastole, the mitral and tricuspid valves are open. and the pulmonary and aortic valves are closed. University Of Saskatchewan Meaning! Blood flows. into the heart throughout diastole thus filling the western university budget atria. and ventricles.

The rate of filling declines as the. ventricles become distended, and the cusps of the. atrioventricular valves start to close. The pressure in the. ventricles remains low throughout late diastole.8. In atrial systole, contraction of the atria forces. additional blood into the ventricles, but approximately 70. percent of the ventricular filling occurs passively during. diastole. Contraction of the atrial muscle that surrounds.

the openings of the superior and university of saskatchewan meaning inferior vena cava and. pulmonary veins, narrows their orifices and the inertia of. the blood moving towards the heart tends to university budget, keep blood in. the heart. However, there is some regurgitation of blood.

into the veins during atrial systole.25. At the start of ventricular systole, the ohio campus scholarships AV valves close. The muscles of the ventricles initially contract relatively. little, but intraventricular pressure rises sharply as the. muscles squeezes the blood in the ventricle. This period of. isovolumetric ventricular contraction lasts about 0.05. seconds until the pressures in the ventricles exceed the. pressure in western michigan budget, the aorta and in the pulmonary artery, and the. aortic and pulmonary valves (semilunar valves) open. During.

this isovolumetric contraction, the AV valves bulge into the. atria, causing a small but sharp rise in atrial pressure. When the semilunar valves open, the stout phase of ventricular. ejection begins. Ejection is initially rapid, but slows. down as systole progresses. The intraventricular pressure. rises to michigan university athletic budget, a maximum and university of wisconsin then declines somewhat before. ventricular systole ends. Late in athletic, systole, the university motto aortic. pressure is actually higher than the western ventricular pressure, but for a short period, momentum keeps the blood moving.

forward. The AV valves are pulled down by the contractions. of the ventricular muscle, and the atrial pressure drops.5. In early diastole, after the ventricular muscle if fully. contracted, the already falling ventricular pressure drops. even more rapidly. This is the period known as. protodiastole and it lasts about 0.04 seconds.

It ends when. the momentum of the ejected blood is overcome and the. semilunar valves close. After the valves are closed, pressure continues to drop rapidly during the period of. isovolumetric relaxation. Isovolumetric relaxation ends. when the ventricular pressure falls below the atrial. pressure and the AV valves open, thus allowing the. ventricles to fill. Again, filling is rapid at first, then.

slows as the next cardiac contraction approaches. Atrial. pressure continues to rise after the end of mein lieblingsbuch essay, ventricular. systole until the AV valves open, upon which time it drops. and slowly rises again until the next atrial systole.6,2,4. The heart is arguably the most vital organ the university budget human body.

possesses. Without the heart, none of the tissues in the. body would receive the vital oxygen necessary for them to. maintain survival. Heart disease is the number one killer. of people in America today. Due to this disturbing fact, it. is no wonder such a large percentage of the fellowships. granted by the National Institutes of Health go towards.

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